Question 1: The breaking of the C-C bonds of complex compounds through oxidation within the cells, leading to release of considerable amount of energy is called?
- Photosynthesis.
- Respiration.
- Transpiration.
- Photorespiration.
Answer: b.
Question 2: Which of the following compounds are oxidized during the process of respiration?
- Respiratory substrates.
- Photosynthetic substrates.
- Inorganic solvents.
- Structural proteins.
Answer: a.
Question 3: In plants, which of the following acts as the primary respiratory substrate?
- Proteins.
- Fats.
- Carbohydrates.
- Organic acids.
Answer: c.
Question 4: What is the main reason plants can get along without respiratory organs?
- Each plant part takes care of its own gas exchange needs.
- Plants do not require oxygen for respiration.
- Plants possess lungs for gaseous exchange.
- Plants utilize carbon dioxide exclusively.
Answer: a.
Question 5: During photosynthesis, oxygen is released within the plant cell. How does this affect respiration?
- It stops cellular respiration.
- It decreases the need for oxygen transport from roots.
- It causes the plant to become an obligate anaerobe.
- It makes oxygen availability an internal feature for leaves.
Answer: d.
Question 6: In woody plants, how does gaseous exchange occur in the thick, woody stems?
- Through stomata.
- Through lenticels.
- Through root hairs.
- Through cuticles.
Answer: b.
Question 7: The complete combustion of glucose yields which of the following as end products?
- Carbon dioxide, water, and energy.
- Lactic acid and energy.
- Ethanol, carbon dioxide, and energy.
- Carbon monoxide, water, and energy.
Answer: a.
Question 8: The strategy of the plant cell to catabolize the glucose molecule in such a way that not all the liberated energy goes out as heat is to oxidize glucose in?
- A single explosive step.
- Several small steps.
- The absence of enzymes.
- The extracellular fluid.
Answer: b.
Question 9: All living organisms retain the enzymatic machinery to partially oxidize glucose without the help of oxygen. This breakdown of glucose to pyruvic acid is called?
- Krebs cycle.
- Glycolysis.
- Electron transport system.
- Calvin cycle.
Answer: b.
Question 10: The term glycolysis has originated from the Greek words, glycos for sugar, and lysis for?
- Synthesis.
- Splitting.
- Joining.
- Reduction.
Answer: b.
Question 11: The scheme of glycolysis was given by which group of scientists?
- Watson, Crick, and Wilkins.
- Gustav Embden, Otto Meyerhof, and J. Parnas.
- Melvin Calvin and Benson.
- Hans Krebs and Fritz Lipmann.
Answer: b.
Question 12: In anaerobic organisms, glycolysis is the only process in respiration. What is its location in the cell?
- Mitochondria.
- Cytoplasm.
- Chloroplast.
- Nucleus.
Answer: b.
Question 13: Sucrose is converted into glucose and fructose by which of the following enzymes?
- Hexokinase.
- Invertase.
- Amylase.
- Zymase.
Answer: b.
Question 14: Glucose and fructose are phosphorylated to give rise to glucose-6-phosphate by the activity of the enzyme?
- Phosphofructokinase.
- Hexokinase.
- Aldolase.
- Pyruvate kinase.
Answer: b.
Question 15: Isomerisation of glucose-6-phosphate produces which of the following intermediates in glycolysis?
- Fructose-6-phosphate.
- Glucose-1-phosphate.
- Fructose-1,6-bisphosphate.
- Phosphoglyceric acid.
Answer: a.
Question 16: How many metabolic steps are present in the glycolytic pathway to convert glucose into pyruvic acid?
- Eight.
- Ten.
- Twelve.
- Fifteen.
Answer: b.
Question 17: ATP is utilized at two steps in glycolysis. The first step is the conversion of glucose into glucose-6-phosphate. What is the second step?
- Fructose-6-phosphate to fructose-1,6-bisphosphate.
- Phosphoenolpyruvate to pyruvic acid.
- 3-phosphoglyceraldehyde to 1,3-bisphosphoglycerate.
- 2-phosphoglycerate to phosphoenolpyruvate.
Answer: a.
Question 18: Fructose-1,6-bisphosphate is split into dihydroxyacetone phosphate and?
- 3-phosphoglyceraldehyde.
- 2-phosphoglycerate.
- 3-phosphoglyceric acid.
- Phosphoenolpyruvate.
Answer: a.
Question 19: During glycolysis, one molecule of NADH + H+ is formed when which of the following is oxidized?
- Glucose-6-phosphate.
- Fructose-1,6-bisphosphate.
- 3-phosphoglyceraldehyde.
- Phosphoenolpyruvate.
Answer: c.
Question 20: The conversion of 1,3-bisphosphoglyceric acid to 3-phosphoglyceric acid is an energy yielding step. This type of ATP synthesis is called?
- Oxidative phosphorylation.
- Photophosphorylation.
- Substrate level phosphorylation.
- Reductive phosphorylation.
Answer: c.
Question 21: Which enzyme catalyzes the conversion of phosphoenolpyruvate to pyruvic acid in glycolysis?
- Enolase.
- Pyruvate kinase.
- Mutase.
- Hexokinase.
Answer: b.
Question 22: What is the net gain of ATP molecules during the glycolysis of one molecule of glucose?
- Two.
- Four.
- Six.
- Eight.
Answer: a.
Question 23: The metabolic fate of pyruvic acid depends on the cellular need. Under anaerobic conditions in yeast, what is the major pathway?
- Lactic acid fermentation.
- Alcoholic fermentation.
- Aerobic respiration.
- Photorespiration.
Answer: b.
Question 24: In alcoholic fermentation, pyruvic acid is converted to ethanol and carbon dioxide by the enzymes pyruvic acid decarboxylase and?
- Lactic dehydrogenase.
- Alcohol dehydrogenase.
- Hexokinase.
- Enolase.
Answer: b.
Question 25: During rigorous exercise, when oxygen is inadequate for cellular respiration in animal muscles, pyruvic acid is reduced to?
- Ethanol.
- Lactic acid.
- Acetic acid.
- Acetyl CoA.
Answer: b.
Question 26: The reducing agent in both lactic acid and alcoholic fermentation is?
- FADH2.
- NADPH.
- NADH + H+.
- ATP.
Answer: c.
Question 27: In both lactic acid and alcoholic fermentation, not much energy is released. Less than what percentage of the energy in glucose is released?
- Seven percent.
- Ten percent.
- Fifteen percent.
- Twenty percent.
Answer: a.
Question 28: Yeasts poison themselves to death when the concentration of alcohol reaches about?
- Five percent.
- Ten percent.
- Thirteen percent.
- Twenty percent.
Answer: c.
Question 29: For the complete oxidation of glucose and extraction of maximal energy, organisms must utilize which process?
- Anaerobic respiration.
- Aerobic respiration.
- Fermentation.
- Glycolysis alone.
Answer: b.
Question 30: Aerobic respiration leads to the complete oxidation of organic substances in the presence of oxygen, and releases?
- Only ATP.
- CO2, water, and a large amount of energy.
- Lactic acid and CO2.
- Ethanol and water.
Answer: b.
Question 31: Pyruvate, which is formed by the glycolytic catabolism of carbohydrates in the cytosol, enters which organelle for further aerobic respiration?
- Nucleus.
- Chloroplast.
- Mitochondrial matrix.
- Endoplasmic reticulum.
Answer: c.
Question 32: The crucial events in aerobic respiration include the stepwise removal of all hydrogen atoms, leaving how many molecules of CO2?
- Two.
- Three.
- Four.
- Six.
Answer: b.
Question 33: The synthesis of ATP via the passing on of electrons to molecular oxygen occurs on the?
- Outer mitochondrial membrane.
- Inner mitochondrial membrane.
- Mitochondrial matrix.
- Cytoplasm.
Answer: b.
Question 34: Pyruvic acid undergoes oxidative decarboxylation in the mitochondrial matrix to produce?
- Citric acid.
- Acetyl CoA.
- Oxaloacetic acid.
- Succinyl CoA.
Answer: b.
Question 35: The reaction catalyzed by pyruvate dehydrogenase requires the participation of several coenzymes, including NAD+ and?
- FAD.
- Coenzyme A.
- ATP.
- Cytochrome c.
Answer: b.
Question 36: During the oxidative decarboxylation of two molecules of pyruvic acid, how many molecules of NADH are produced?
- One.
- Two.
- Three.
- Four.
Answer: b.
Question 37: Acetyl CoA enters a cyclic pathway known as the tricarboxylic acid cycle, more commonly called?
- Calvin cycle.
- Glyoxylate cycle.
- Krebs cycle.
- Cori cycle.
Answer: c.
Question 38: The first step of the TCA cycle is the condensation of acetyl group with oxaloacetic acid (OAA) and water to yield?
- Isocitric acid.
- Citric acid.
- Alpha-ketoglutaric acid.
- Succinic acid.
Answer: b.
Question 39: The enzyme that catalyzes the condensation of acetyl CoA and OAA to form citric acid is?
- Citrate synthase.
- Aconitase.
- Isocitrate dehydrogenase.
- Malate dehydrogenase.
Answer: a.
Question 40: Citrate is isomerised to isocitrate. This is followed by two successive steps of decarboxylation, leading to the formation of?
- Malic acid and oxaloacetic acid.
- Alpha-ketoglutaric acid and succinyl-CoA.
- Fumaric acid and malic acid.
- Succinic acid and fumaric acid.
Answer: b.
Question 41: During the conversion of succinyl-CoA to succinic acid, a molecule of GTP is synthesized. This is an example of?
- Oxidative phosphorylation.
- Substrate level phosphorylation.
- Photophosphorylation.
- Reductive amination.
Answer: b.
Question 42: In a coupled reaction during the TCA cycle, GTP is converted to GDP with the simultaneous synthesis of?
- AMP from ADP.
- ATP from ADP.
- CTP from CDP.
- UTP from UDP.
Answer: b.
Question 43: In one turn of the TCA cycle, how many points are there where NAD+ is reduced to NADH + H+?
- One.
- Two.
- Three.
- Four.
Answer: c.
Question 44: In the TCA cycle, FAD is reduced to FADH2 at how many specific points?
- One.
- Two.
- Three.
- Four.
Answer: a.
Question 45: The continued oxidation of acetyl CoA via the TCA cycle requires the continuous replenishment of which compound?
- Pyruvic acid.
- Oxaloacetic acid.
- Citric acid.
- Malic acid.
Answer: b.
Question 46: What is the primary role of the electron transport system (ETS) in the mitochondria?
- To synthesize glucose from carbon dioxide.
- To oxidize NADH and FADH2 and pass electrons to oxygen.
- To produce carbon dioxide from pyruvate.
- To transport ATP out of the mitochondria.
Answer: b.
Question 47: Electrons from NADH produced in the mitochondrial matrix are oxidized by which complex of the ETS?
- Complex II.
- Complex I.
- Complex III.
- Complex IV.
Answer: b.
Question 48: The electrons from Complex I are transferred to which mobile electron carrier located within the inner membrane?
- Cytochrome c.
- Ubiquinone.
- Plastocyanin.
- Plastoquinone.
Answer: b.
Question 49: Ubiquinone also receives reducing equivalents via FADH2, which is associated with which complex?
- Complex I.
- Complex II.
- Complex III.
- Complex IV.
Answer: b.
Question 50: The reduced ubiquinone (ubiquinol) is oxidized with the transfer of electrons to cytochrome c via which complex?
- Complex I.
- Complex II.
- Cytochrome bc1 complex (Complex III).
- Cytochrome c oxidase (Complex IV).
Answer: c.
Question 51: Cytochrome c is a small protein attached to the outer surface of the inner membrane and acts as a mobile carrier for transfer of electrons between?
- Complex I and II.
- Complex II and III.
- Complex III and IV.
- Complex I and III.
Answer: c.
Question 52: Complex IV of the ETS is also known as cytochrome c oxidase. It contains cytochromes a and a3, and two centers of which metal?
- Iron.
- Copper.
- Zinc.
- Magnesium.
Answer: b.
Question 53: As electrons pass from one carrier to another via complex I to IV in the ETS, they are coupled to ATP synthase for the production of ATP. Which complex is ATP synthase?
- Complex II.
- Complex III.
- Complex IV.
- Complex V.
Answer: d.
Question 54: The oxidation of one molecule of NADH gives rise to how many molecules of ATP through the ETS?
- One.
- Two.
- Three.
- Four.
Answer: c.
Question 55: The oxidation of one molecule of FADH2 through the electron transport system produces how many molecules of ATP?
- One.
- Two.
- Three.
- Four.
Answer: b.
Question 56: The role of oxygen in aerobic respiration is crucial because it acts as the final acceptor of?
- Carbon.
- Hydrogen.
- ATP.
- Water.
Answer: b.
Question 57: In respiration, the energy of oxidation-reduction is utilized for the production of proton gradient required for phosphorylation. This process is called?
- Photophosphorylation.
- Substrate level phosphorylation.
- Oxidative phosphorylation.
- Reductive phosphorylation.
Answer: c.
Question 58: According to the chemiosmotic hypothesis, what drives the synthesis of ATP by ATP synthase?
- A gradient of sodium ions.
- The movement of electrons.
- A proton gradient across the inner membrane.
- The breakdown of water.
Answer: c.
Question 59: Complex V (ATP synthase) consists of two major components, F1 and F0. What is the role of the F1 headpiece?
- It acts as a proton channel.
- It is the site for synthesis of ATP from ADP and Pi.
- It binds oxygen.
- It transports electrons.
Answer: b.
Question 60: The F0 component of ATP synthase is an integral membrane protein complex that forms the channel through which?
- Electrons cross the membrane.
- Protons cross the inner membrane.
- ATP exits the mitochondria.
- Water enters the matrix.
Answer: b.
Question 61: For each ATP produced, how many protons pass through F0 from the intermembrane space to the matrix down the electrochemical proton gradient?
- One.
- Two.
- Three.
- Four.
Answer: b.
Question 62: To calculate the net gain of ATP for every glucose molecule oxidized, it is assumed that the NADH synthesized in glycolysis is transferred into the mitochondria and undergoes?
- Reduction.
- Fermentation.
- Oxidative phosphorylation.
- Substrate level phosphorylation.
Answer: c.
Question 63: In reality, the respiratory pathway is not a strictly linear sequence because intermediates are?
- Never utilized for other pathways.
- Withdrawn to synthesize other compounds as needed.
- Destroyed immediately after formation.
- Locked within the mitochondria permanently.
Answer: b.
Question 64: A net gain of how many ATP molecules is theoretically possible during aerobic respiration of one molecule of glucose?
- 2 ATP.
- 18 ATP.
- 38 ATP.
- 48 ATP.
Answer: c.
Question 65: How does the energy yield of fermentation compare to that of aerobic respiration?
- Fermentation yields more energy.
- They yield the same amount of energy.
- Fermentation accounts for only a partial breakdown of glucose, yielding less energy.
- Fermentation yields twice as much energy.
Answer: c.
Question 66: NADH is oxidized to NAD+ rather slowly in fermentation. How is this oxidation in aerobic respiration?
- Very slow.
- Extremely slow.
- Non-existent.
- Very vigorous.
Answer: d.
Question 67: Respiratory pathways are primarily catabolic, but since they also provide intermediates for biosynthesis, they are better considered as?
- Anabolic pathways.
- Amphibolic pathways.
- Endergonic pathways.
- Non-metabolic pathways.
Answer: b.
Question 68: If fats are to be respired, they must first be broken down into?
- Glycerol and fatty acids.
- Amino acids.
- Glucose and fructose.
- Pyruvate and lactate.
Answer: a.
Question 69: Before entering the respiratory pathway, fatty acids are degraded into which compound?
- Pyruvic acid.
- Acetyl CoA.
- Alpha-ketoglutaric acid.
- Phosphoglyceraldehyde.
Answer: b.
Question 70: Glycerol would enter the respiratory pathway after being converted to?
- Phosphoglyceraldehyde (PGAL).
- Phosphoenolpyruvate.
- Citric acid.
- Lactic acid.
Answer: a.
Question 71: Proteins are degraded by proteases into amino acids, which enter the respiratory pathway after undergoing?
- Phosphorylation.
- Deamination.
- Decarboxylation.
- Polymerization.
Answer: b.
Question 72: An amino acid after deamination may enter the TCA cycle as pyruvic acid, acetyl CoA, or?
- Another stage within the Krebs cycle.
- Cytochrome c.
- Glucose-6-phosphate.
- Fructose-1,6-bisphosphate.
Answer: a.
Question 73: The ratio of the volume of CO2 evolved to the volume of O2 consumed in respiration is called the?
- Respiratory rate.
- Respiratory Quotient (RQ).
- Photosynthetic quotient.
- Metabolic ratio.
Answer: b.
Question 74: The Respiratory Quotient (RQ) depends heavily upon the?
- Temperature of the environment.
- Type of respiratory substrate used.
- Amount of water available.
- Light intensity.
Answer: b.
Question 75: When carbohydrates are used as substrate and are completely oxidized, what is the RQ value?
- 0.7.
- 0.9.
- 1.0.
- More than 1.
Answer: c.
Question 76: When fats are used in respiration, the RQ is?
- Less than 1.
- Equal to 1.
- More than 1.
- Infinity.
Answer: a.
Question 77: The calculated RQ for tripalmitin, a fatty acid, is approximately?
- 1.0.
- 0.9.
- 0.7.
- 1.3.
Answer: c.
Question 78: When proteins are the respiratory substrates, the RQ would be about?
- 0.7.
- 0.9.
- 1.0.
- 1.5.
Answer: b.
Question 79: In living organisms, are respiratory substrates strictly one type of molecule at a time?
- Yes, only carbohydrates are ever used.
- Yes, only fats are used.
- No, rarely is a single type of substrate used; often a mixture is utilized.
- Pure proteins are used exclusively in plants.
Answer: c.
Question 80: Which statement about pure proteins or fats as respiratory substrates is correct?
- They are never used as respiratory substrates under normal conditions.
- They enter the pathway without modification.
- They yield more CO2 than O2 consumed.
- They have an RQ greater than 1.
Answer: a.
Question 81: Which intermediate of the TCA cycle is used for the synthesis of chlorophyll, cytochromes, and phytochrome?
- Citric acid.
- Succinyl CoA.
- Oxaloacetic acid.
- Alpha-ketoglutaric acid.
Answer: b.
Question 82: Which of the following is an intermediate of glycolysis that can be used for the synthesis of glycerol?
- Phosphoenolpyruvate.
- 3-phosphoglyceraldehyde.
- Dihydroxyacetone phosphate.
- Pyruvic acid.
Answer: c.
Question 83: To withdraw oxaloacetic acid for the synthesis of pyrimidines and alkaloids, the Krebs cycle is considered an?
- Amphibolic pathway.
- Anabolic pathway exclusively.
- Anaerobic pathway.
- Irreversible pathway.
Answer: a.
Question 84: The inner mitochondrial membrane has invaginations called cristae, which serve to?
- Increase surface area for ETS complexes.
- Store genetic material.
- Synthesize glucose.
- Trap light energy.
Answer: a.
Question 85: Cyanide is a potent poison that halts respiration by inhibiting which complex of the electron transport system?
- Complex I.
- Complex II.
- Complex III.
- Complex IV (cytochrome c oxidase).
Answer: d.
Question 86: What is the main difference between substrate-level phosphorylation and oxidative phosphorylation?
- Substrate-level needs oxygen, oxidative does not.
- Substrate-level uses a proton gradient, oxidative uses enzyme substrates directly.
- Substrate-level directly transfers a phosphate from a substrate to ADP, oxidative uses an electrochemical gradient.
- Oxidative phosphorylation occurs in the cytoplasm, substrate-level in mitochondria.
Answer: c.
Question 87: During the complete oxidation of a glucose molecule, how many molecules of water are formed as a metabolic byproduct?
- 2.
- 4.
- 6.
- 12.
Answer: c.
Question 88: In the conversion of pyruvic acid to acetyl CoA, which specific gas is released?
- Oxygen.
- Nitrogen.
- Carbon dioxide.
- Carbon monoxide.
Answer: c.
Question 89: Which step is commonly known as the connecting link between glycolysis and the Krebs cycle?
- Conversion of PEP to Pyruvate.
- Conversion of Pyruvate to Acetyl CoA.
- Conversion of Acetyl CoA to Citrate.
- Conversion of Citrate to Isocitrate.
Answer: b.
Question 90: How many turns of the Krebs cycle are required for the complete oxidation of one molecule of glucose?
- One.
- Two.
- Three.
- Four.
Answer: b.
Question 91: Identify the correct sequence of electron carriers in the cytochrome bc1 complex.
- Cytochrome b -> Fe-S -> Cytochrome c1.
- Cytochrome c1 -> Fe-S -> Cytochrome b.
- Fe-S -> Cytochrome b -> Cytochrome c1.
- Cytochrome b -> Cytochrome c1 -> Fe-S.
Answer: a.
Question 92: Which of the following is NOT a characteristic feature of fermentation?
- Incomplete oxidation of glucose.
- Net gain of only 2 ATP molecules.
- Slow oxidation of NADH to NAD+.
- Requires the presence of a functional electron transport chain.
Answer: d.
Question 93: If the RQ value is less than 1, it implies that?
- More carbon dioxide is evolved than oxygen consumed.
- More oxygen is consumed than carbon dioxide evolved.
- The substrate is a carbohydrate.
- Respiration is anaerobic.
Answer: b.
Question 94: Malic acid is a 4-carbon compound. How many decarboxylation steps occur between citric acid and malic acid in the TCA cycle?
- None.
- One.
- Two.
- Three.
Answer: c.
Question 95: Which of the following enzymes of the TCA cycle is located in the inner mitochondrial membrane rather than the matrix?
- Citrate synthase.
- Alpha-ketoglutarate dehydrogenase.
- Succinate dehydrogenase.
- Malate dehydrogenase.
Answer: c.
Question 96: During rigorous exercise, the accumulation of which compound in the muscle cells causes fatigue?
- Pyruvic acid.
- Lactic acid.
- Carbon dioxide.
- Ethanol.
Answer: b.
Question 97: Fructose-1,6-bisphosphate aldolase splits a 6-carbon sugar into two 3-carbon sugars. This enzyme belongs to which class of enzymes?
- Oxidoreductases.
- Transferases.
- Lyases.
- Ligases.
Answer: c.
Question 98: Oxygen acts as the final hydrogen acceptor. Its role is restricted to which stage of respiration?
- The terminal stage.
- The initial stage of glycolysis.
- The Krebs cycle.
- The Link reaction.
Answer: a.
Question 99: If a mutant plant cell lacks Complex III, which of the following processes will immediately stop?
- Glycolysis.
- Formation of acetyl CoA.
- Flow of electrons from ubiquinol to cytochrome c.
- Conversion of PEP to pyruvate.
Answer: c.
Question 100: Which of the following statements best describes the term 'respiratory balance sheet'?
- A calculation of oxygen inhaled versus exhaled.
- A theoretical calculation of net ATP yield based on sequential assumptions.
- An exact measurement of ATP in a living cell.
- A record of water loss during transpiration.
Answer: b.


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